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The data and information presented in this web site are presented in good faith and believed to be accurate. Any and all liability for the content or any omissions including any inaccuracies, errors, or misstatements in such data or information is expressly disclaimed. The web site is compiled for the sole purpose of informing community members of resources and information pertaining to Lyme Borreliosis Disease and its coinfections.
The Canadian Lyme Disease Foundation, Directors and members are not liable for any direct or indirect damages or any damages whatsoever resulting from loss of use, data or profits, whether in an action of contract, negligence or other tortious action arising out of or in connection with the use or performance of information available from this website.
Consult a qualified Lyme ( Borreliosis ) Disease literate doctor for medical advice if Lyme Disease is suspect.
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This is an abstract presented by Dr. Bach at the International Scientific Conference
on Lyme Disease, April, 2001.
RECOVERY OF LYME SPIROCHETES BY PCR IN SEMEN SAMPLES OF PREVIOUSLY
DIAGNOSED LYME DISEASE PATIENTS
Dr. Gregory Bach, Do.O., P.C. 2415 North Broad Street, Colmar, PA 18915
OBJECTIVE
Lyme disease, being a spirochete with pathology similar to syphilis, is often found
difficult to treat due to the spirochete invading sanctuary sites and displaying
pleomorphic characteristics such as a cyst (L-form). Because a significant portion of
sexually active couples present to my office with Lyme disease, with only one
partner having a history of tick exposure, the question of possible secondary
(sexual)vector of transmission for the spirochete warrents inquiriy.
Additionally,
sexually active couples seem to have a marked propensity for antibiotic failure
raising the question of sexually active couples re-infecting themselves through
intimate contact.
METHODS:
Lyme spirochetes/DNA have been recovered from stored animal
semen. Recovery of spirochete DNA from nursing mother's breast milk and unbilical
cord blood by PCR (confirmed by culture/microscopy), have been found in samples
provided to my office.
RESULTS:
Suprisingly, initial laboratory testing of semen samples provided by
male Lyme patients (positive by western blot/PCR in blood) and the male sexual
partner of a Lyme infected female patient were positive approximately 40% of the
time. PCR recovery of Lyme DNA nucleotide sequences with microscopic
confirmation of semen samples yielded positive results in 14/32 Lyme patients (13
male semen samples and 1 vaginal pap).
ALL positive semen/vaginal samples in
patients with known sexual partners resulted in positive Lyme titers/PCR in their
sexual partners. 3/4 positive semen patients had no or unknown sexual partners to
be tested. These preliminary findings warrent futher study. Current a statistical
design study to evaluate the possibility of sexual transition of the spirochete is
being undertaken.
Our laboratory studies confirm the existence of Lyme spirochetes
in semen/vaginal secretions. Whether or not further clinical studies with a larger
statistical group will support the hypothesis of sexual transmission remains to be
seen. A retrospective clinical study is also underway.
We are reviewing the medical
records, collecting semen samples of patients who were previously diagnosed with
current and previously treated Lyme disease are bing asked to provide semen,pap
and blood samples for extensive laboratory testing.
CONCLUSION:
With the initially impressive data, we feel the subsequent
statistical sudy on the sexual transmission of the Lyme spirochete will illuminate a
much broader sectrum of public health concerns associated with the disease than
the originally accepted tick borne vector.
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