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Lyme Disease symptoms vary from person to person. (lymes disease lyme's disease lime disease limes disease)
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Isolation of Borrelia afzelii from overwintering Culex pipiens biotype
molestus mosquitoes.
Ann Agric Environ Med. 2006;13(2):345-8.
* Zakovska A,
* Capkova L,
* Sery O,
* Halouzka J,
* Dendis M.
Department of Comparative Animal Physiology and General Zoology, Faculty
of Science, Masaryk University, Kotlarska 2, CZ-611 37 Brno, Czech Republic;
alenazak@sci.muni.cz.
A total of 662 samples (winter period: 469; summer period: 193
specimens) of female mosquitoes of the genus Culex, Aedes and Anopheles were
collected during the period March 2000-April 2001 from the locality of
Vysoke Myto (Eastern Bohemia, Czech Republic). They were examined by dark
field microscopy for the presence of spirochetes. The motile spirochetes
were observed in 4.2 % of all species of investigated mosquitoes. One
spirochetal strain out of the 8 isolation attempts (BRZ14) was obtained
(cultivation rate was 12.5 %) and the spirochetal strain was then
successfully cultivated and identified using PCR for the presence of
Borrelia burgdorferi s.l., and subsequently with the RFLP as genomospecies
Borrelia afzelii. This strain was derived from overwintering Culex (Culex)
pipiens biotype molestus female mosquitoe. This is apparently one of the
sporadic cases of the occurrence of pathogenic borreliae in haematophagous
arthropods, other than Ixodes ricinus complex ticks.
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