Or...Search this site Home Symptoms Live Discussion Diagnosis Treatment Area Support Library Research Lymelinks Contact Pets & Lyme DONATIONS Drug Info Medical Dictionary Board of Directors      Click on the graphic to vote for this site as a Starting Point Hot Site. -- No Warranties or Representations The data and information presented in this web site are presented in good faith and believed to be accurate. Any and all liability for the content or any omissions including any inaccuracies, errors, or misstatements in such data or information is expressly disclaimed. The web site is compiled for the sole purpose of informing community members of resources and information pertaining to Lyme Borreliosis Disease and its coinfections. The Canadian Lyme Disease Foundation, Directors and members are not liable for any direct or indirect damages or any damages whatsoever resulting from loss of use, data or profits, whether in an action of contract, negligence or other tortious action arising out of or in connection with the use or performance of information available from this website. Consult a qualified Lyme ( Borreliosis ) Disease literate doctor for medical advice if Lyme Disease is suspect. en français For Physicians Ticks Coinfections Prevention Our Stories Click Here to order our free Lyme Disease Flyer,    Here for our free Lyme Disease Poster ..documents may be copied (to distribute) but edit only for alignment. A Rapid Method for Generating Cystic Forms of Borrelia burgdorferi, and Their Reversal to Mobile Spirochetes Date of Publication: December, 1998 Source: APMIS, 106(12):1131-1141 Authors: (1) Brorson O; (2) Brorson S. Institution: (1) Department of Microbiology, Vestfold Sentralsykehus, Tonsberg (2) Department of Pathology, Ulleval Hospital, Oslo, Norway Abstract Mobile Borrelia burgdorferi were transferred to distilled water (106 per ml). The cultures were observed by dark field microscopy (DFM), interference contrast microscopy (ICM) and transmission electron microscopy (TEM). 95% of the spirochetes were converted to cysts after 1 min, and after 4 h no normal mobile borreliae were observed. When transferred to growth medium (BSK-H), the cysts became smaller and more irregular, and were filled with organic substances. After 1 day, 1-5 thin structures sprouted from the cysts. They continued to grow in both length and thickness until they attained a normal spirochetal structure. Finally, these new-born spirochetes detached from the cysts, by which time their mobility had become normal. The present method for producing large amounts of cystic forms of B. burgdorferi is well suited for further studies of this unique microbe. Quotation From The Full-Text Article "...cystic forms may occur in the human organism (11,14,15), and they may explain the long periods of latency, resistance to antibiotics, negative serological results (3-7,10,12,13,28), and low PCR sensitivity (5,8,10). (p.1139)"