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Lyme Disease symptoms vary from person to person. (lymes disease lyme's disease lime disease limes disease)
The data and information presented in this web site are presented in good faith and believed to be accurate regarding Lyme disease (commonly misspelled lymes disease lyme's disease lime disease limes disease) and other related diseases. Any and all liability for the content or any omissions including any inaccuracies, errors, or misstatements in such data or information is expressly disclaimed. The web site is compiled for the sole purpose of informing community members of resources and information pertaining to Lyme Borreliosis Disease and its coinfections. Lyme disease symptoms may vary from person to person.
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Consult a qualified Lyme ( Borreliosis ) Disease literate doctor for medical advice if Lyme Disease is suspect to discuss your Lymes Disease Symptoms.
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HrpA, a DEAH-Box RNA Helicase, Is Involved in Global Gene Regulation in
the Lyme Disease Spirochete.
Salman-Dilgimen A, Hardy PO, Dresser AR, Chaconas G
PLoS ONE 2011 08; 6 (7)
Spirochetes causing Lyme borreliosis are obligate parasites that can
only be found in a tick vector or a vertebrate host. The ability to
survive in these two disparate environments requires up and
downregulation of specific genes by regulatory circuits that remain
largely obscure.
In this work on the Lyme spirochete, B. burgdorferi, we
show that a disruption of the hrpA gene, which encodes a putative RNA
helicase, results in a complete loss in the ability of the spirochetes
to infect mice by needle inoculation. Studies of protein expression in
culture by 2D gels revealed a change in the expression of 33 proteins in
hrpA clones relative to the wild-type parent. Quantitative
characterization of protein expression by iTRAQ analysis revealed a
total of 187 differentially regulated proteins in an hrpA background: 90
downregulated and 97 upregulated. Forty-two of the 90 downregulated and
65 of the 97 upregulated proteins are not regulated under any conditions
by the previously re ported regulators in B. burgdorferi (bosR, rrp2,
rpoN, rpoS or rrp1). Downregulated and upregulated proteins also fell
into distinct functional categories.
We conclude that HrpA is part of a
new and distinct global regulatory pathway in B. burgdorferi gene
expression. Because an HrpA orthologue is present in many bacteria, its
participation in global regulation in B. burgdorferi may have relevance
in other bacterial species where its function remains obscure. We
believe this to be the first report of a role for an RNA helicase in a
global regulatory pathway in bacteria. This finding is particularly
timely with the recent growth of the field of RNA regulation of gene
expression and the ability of RNA helicases to modulate RNA structure
and function.
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