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Lyme Disease symptoms vary from person to person. (lymes disease lyme's disease lime disease limes disease)
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Focus floating microscopy: "gold standard" for cutaneous borreliosis?
Am J Clin Pathol. 2007 Feb;127(2):1-10.
Eisendle K, Grabner T, Zelger B.
Department of Dermatology and Venerology, Innsbruck Medical University,
Innsbruck, Austria.
Borrelia burgdorferi is difficult to detect in routine biopsy material from
patients with skin lesions of borreliosis.
In this study, a new immunohistochemical method, focus floating microscopy (FFM), was developed to
detect B burgdorferi in tissue sections and was compared with polymerase chain
reaction (PCR).
By using standard histologic equipment, tissue sections stained
with a polyclonal B burgdorferi antibody were simultaneously scanned through 2
planes: horizontally in serpentines and vertically by focusing through the
thickness of the section.
Borrelia were detected in 47 of 71 ticks, 34 of 66 tick
bites, 30 of 32 erythema chronicum migrans cases, 41 of 43 borrelial
lymphocytomas, and 50 of 51 acrodermatitis chronica atrophicans cases. FFM
proved to be more sensitive than PCR (96.0% vs 45.2%) and nearly equally
specific (99.4% vs 100%). All 169 control cases, except 1 false-positive case of
secondary syphilis, were negative with FFM.
FFM is an easy, quick, and
inexpensive method to reliably detect Borrelia in cutaneous tissue sections.
Full article
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